Re: [Histonet] Zinc.
I've been forced to use a zinc only fixative (Streck Tissue Fixative) for
a couple of years - BD (Pharmingen) recommends the use of this for many of
it's antibodies - i.e. Caspase-3 and CD31 - works great, but the
morphology stinks. I'm in the process of 'crossing over' to find
antibodies that will work on FFPE as well on my xenografts.
Jackie O'
Ian Montgomery
Sent by: histonet-bounces@lists.utsouthwestern.edu
11/05/2004 08:54 AM
To: histonet@lists.utsouthwestern.edu
cc:
Subject: [Histonet] Zinc.
When choosing an antibody the companies usually give the
preferred
method of preparation, frozen, formalin, zinc etc. When they say zinc, do
they mean a zinc only fixative or a zinc formalin mixture?
Ian.
Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery@bio.gla.ac.uk
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