Re: [Histonet] Zinc.

From:"Jackie M O'Connor"

I've been forced to use a zinc only fixative (Streck Tissue Fixative) for 
a couple of years - BD (Pharmingen) recommends the use of this for many of 
it's antibodies - i.e. Caspase-3 and  CD31 - works great, but the 
morphology stinks.  I'm in the process of 'crossing over' to find 
antibodies that will work on FFPE as well on my xenografts. 
Jackie O'




Ian Montgomery 
Sent by: histonet-bounces@lists.utsouthwestern.edu
11/05/2004 08:54 AM

 
        To:     histonet@lists.utsouthwestern.edu
        cc: 
        Subject:        [Histonet] Zinc.


         When choosing an antibody the companies usually give the 
preferred 
method of preparation, frozen, formalin, zinc etc. When they say zinc, do 
they mean a zinc only fixative or a zinc formalin mixture?
Ian.

Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery@bio.gla.ac.uk 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>