Ask your client if fat can be washed out
by water?  You and I know it can't. 
Persuade your client to buy a textbook
and join the Histochemical Society ($50
per year, with a journal arriving every
month!)

John Kiernan
London, Canada.  
------------------------------------
Andrea Grantham wrote:
> 
> This may be a crazy question. I'm doing mouse livers for a lab here and
> they requested Oil Red O. Did the stain - looked great. They are asking if
> it was possible that some of the fat washed away during the washing steps.
> The experiment requires that they quantitate the fat found in these
> sections. Is this a possibility? Is there a better way to quantitate fat?
> For the record - my washing steps are done under a gentle stream of tap
> water running into a corner of the stain dish or coplin jar - not a full
> force gush of water from the faucet directly on the slides.
> Thanks.
> Andi Grantham
> .....................................................................
> : Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
> : Sr. Research Specialist       University of Arizona               :
> : (office:  AHSC 4212)          P.O. Box 245044                     :
> : (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
> : (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
> :...................................................................:
>            http://www.cba.arizona.edu/histology-lab.html
> 
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