Re: [Histonet] LacZ Fixative

From:ajennings@unmc.edu





Years of experience with rodent tissues expressing B-Gal. I have had no
problems with formalin fixed samples and looking at B-Gal. There was an
article in JHC that compared a few fixatives where time was considered one
of the most important factors, and glutaraldehyde was the chosen fixative.
Journal of Histochemistry and Cytochemistry, Vol. 50, 1421-1424, October
2002, Copyright  2002, The Histochemical Society, Inc.




Hello,
Can anyone with experience recommend the best fixative for the B-Gal
procedure for
the LacZ reporter gene in rodent tissues?
Thanks.
Lin
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