Re: [Histonet] IHC for Microglia

From:Sharon Cooperman

There are two antibodies I know of that work on ffpe macrophages 
including microglia.  They are ED-1 (Serotec) (requires pH 6.0 
citrate antigen retrieval, I use a microwave pressure cooker) and 
Mac-3 (Pharmingen) (supposedly doesn't require antigen retrieval, 
hasn't been as strong staining as ED-1 but I haven't really tried 
that hard).  They both stain golgi, so theoretically stain other cell 
types as well, but in reality only stain macrophages so you can see 
it and it seems to be well accepted in the literature that they are 
macrophage-specific markers.


>My colleague is trying to find an antibody that is specific for 
>microglial cells that will work on formalin fixed, paraffin embedded 
>mouse brain sections. Has any one had any luck with a particular 
>Histonet mailing list

Sharon Cooperman       	     
NIH, NICHD, CBMB                     301.435-8417
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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