Re: [Histonet] Antibody Help microphthalmia and CD34

From:"Richard Cartun"

A 1:10 dilution of an antibody that probably costs a few hundred dollars
doesn't sound very economical.  How long are you incubating?


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Mildred Fail"  11/09/04 04:36PM >>>
We use CD34 from Becton-Dickinson, at 1:100, Hier with Citrate buffer
and MITF from Novocastra, 1:10, HIER with Citrate Buffer
Rena Fail
Medical  University of SC
Charleston, SC

>>> Caldwell  11/09/04 12:58PM >>>
Hi Everyone,
I am looking for a good antibody for CD34 and microphthalmia-associated
transcription factor.  We have tried a couple of vendors for the CD34,
and the pathologist hasn't been happy with them.
Any suggestions would be greatly appreciated.  Thank you in advance for
your help.
Louri Roberts-Caldwell

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