Wayne,

You may want to explore the expertise of the dept of neuropathology at
Harborview in your hometown.  Two individuals in particular.  One of the
neuropathologists, Dr. Donald E. Born, M.D. and the lead neuropathology
histotechnologist, Christiana, of who's specialties include muscle biopsies.
One of their specimen prep techniques includes flash freezing muscle in
2-Methylbutane (cooled by liquid nitrogen) 
@ -160 degrees C.  In theory this minimizes morphological distortion of the
specimen by rapidly forcing the water component of the tissue to change from
a liquid to a gas without passing through a solid state (my inorganic
chemistry classes are way too far in the past to allow me to recall the name
for this physical process), but thought this might have some applicability
to or lead you down a new pathway in the work you're doing.  Good luck,
Greg

Greg Luck, BS, HT(ASCP)
Anatomic Pathology Supervisor
Deaconess Medical Center
800 W. 5th Ave
Spokane, WA 99204
Phone 509.473.7077
Fax 509.473.7133
luckg@empirehealth.org




-----Original Message-----
From: Wayne Kreider [mailto:wkreider@u.washington.edu]
Sent: Monday, November 01, 2004 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] sample preparation/analysis to observe gas bubbles


We're exploring a treatment in which small short-lived (perhaps on the 
order of seconds) gas bubbles may be generated in tissue.  To this end, 
we're interested in learning the 'instantaneous' state of the tissue 
immediately after treatment with regard to the presence of any bubbles.  
Currently, our experiments utilize rabbit muscle.  Are there any known 
histological sample preparation/analysis procedures that have been used 
to preserve/observe resident gas bubbles?  We have considered a flash 
freezing followed by staining and/or electron microscopy.  However, our 
expertise is not in histology, so we'd very much appreciate any 
suggestions and/or details regarding possible sample preparation.  Thanks.
Wayne
-- 

/**********************/

/Wayne Kreider/

/University of Washington/

/Applied Physics Lab / CIMU/

/106 Old Fisheries/

/206-543-1324/

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