RE: [Histonet] restoring dried tissue
I have always used Ruffers solution, and it works, there are many vaiants of the formula, but your tissue can be restored. Ruffer worked with Egyptian mummies tissue, so if it was good enough for them it should be fine for your tissue.i am attaching my recipe
holy cross hospital
ft lauderdale, fl
From: Julienne Rutherford [mailto:firstname.lastname@example.org]
Sent: Friday, November 12, 2004 10:53 AM
To: Histonet@Lists. Utsouthwestern. Edu
Subject: [Histonet] restoring dried tissue
I work with marmoset monkeys. I have some dried placental tissue - nobody to
observe the birth and collect the placenta, so tissue dried out overnight.
Is there a way rehydrate dried tissue prior to fixation in formalin, or
should I just write it off? I also have some tissue that was collected some
years ago that was found in the same opportunistic way, dessicated but
placed in formalin as is. Placenta jerky. What can be done with tissue like
this? I'm planning to do H & E on my placental samples and am interested in
doing IHC for IGF.
Julienne Rutherford, M.A.
Southwest Foundation for Biomedical Research
San Antonio, TX
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