RE: [Histonet] a much easier chemistry question

From:"Weems, Joyce"

And that would be ERADO-SOL	C6347It really does work! jJoyce WeemsPathology ManagerSaint Joseph's Hospital 5665 Peachtree Dunwoody Rd NEAtlanta, GA 30342404-851-7376 - Phone404-851-7831 - Fax-----Original Message-----From:[]On Behalf Of FordRoyerSent: Friday, November 05, 2004 12:37 PMTo: Histonet@lists.utsouthwestern.eduSubject: Re: [Histonet] a much easier chemistry questionYears ago, Scientific Products made a lanolin-based cream stain remover that worked great in removing biological stains.  It worked like magic on stain on hands and lab surfaces.  One day, out of frustration, I tried it on my white lab coat (stained with methylene blue) and it worked fine.  I rubbed the cream into the stain, and then washed to coat in the washing machine... stain all gone!  I check a recent (1997) Allegiance catalog, and they still showed this product.  Called "Dye-Sol" and the catalog number was C6345.  All the S/P products are now carried by Cardinal so you could contact them to see if they still carry it.  They should be able to cross-reference the old Allegiance cat. no.Good Luck!~ FordFord M. Royer, MT(ASCP)Midwest Science BiocenterMinneapolis, MNJohn Kiernan wrote:>Your unsuccessful efforts were all acidic, >neutral or hydrophobic. Trypan and Evans blue are >anionic dyes with large, very hydrophilic molecules.>Alkaline solutions extract anionic dyes, and urea >(high concentration) can disrupt the non-ionic, >non-covalent bonding that holds large hydrophilic>dye molecules to substrates such as cellulose (cotton). >>Try a strong urea solution (8M=48%) made alkaline>with borax or washing soda. Geoff McAuliffe's>simpler suggestion of soap and water may work,>because soap is alkaline. >>Keep us informed. All who use dyes need this sort>of information. (I have a grey tie with alcian >blue spots dating from the 1960s; wouldn't want to >remove them now, though I'm sure it would be>impossible without dissolving the fabric.)>>John Kiernan>London, Canada>------------------------------------->Jack England wrote:>  >>>Aloha all,>>>>Here's a much more fun chemistry question for anyone that wants to bite:>>what is the best method to remove trypan/evans blue from cotton fabric?  One>>of my colleagues got some on her pants the other day and asked what the best>>way to get it out was.  I did not know, so I put some on cotton gauze, and>>tried:>>>>--acid alcohol...faded the stain a little, but didn't get rid of it>>--DI change>>--absolute change>> change>>--ethanol/non-ionic detergent/DI water change>>--citrus oil change>>>>Given that her fabric is tan (and thus not white, and thus likely not>>bleach-able), can anyone out there in histo-land suggest a way of>>de-staining trypan-blue-stained fabric? I've been wondering about this for a>>while now and figured I'd pass it around.>>>>--Many thanks and aloha to all,>>Jack England>>Tissue Genesis, Inc.>>>>>Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer.Thank you. Saint Josephs Health System, Inc.

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