RE: [Histonet] HMB50 and D2-40

From:"Bill Sinai"


I have spoken to the lady who does ourIHC on the Benchmark and she uses the
HMB45 + 50 antibody from Neomarkers.  She uses a mild CC1, the antibody
diluted 1:50 and a 32min incubation time.

Hope this helps.

What is the other D2-40 antibody?

Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Ph 02 9845 7774

-----Original Message-----
[]On Behalf Of B T
Sent: Friday, 05 November 2004 8:26 AM
Subject: [Histonet] HMB50 and D2-40

Hi all,

Is anyone out there doing melanoma clone HMB50 on the Ventana Benchmark? If
so, what protocol are you using? I am having no success working up this
antibody and at this point i'm not sure if there might be a problem eith the
antibody itself.

Also, is anyone using D2-40 on the Benchmark......I am getting staining but
would like to punch it up a notch without having the tissue fall off the

Help please!


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