bARB,

I have spoken to the lady who does ourIHC on the Benchmark and she uses the
HMB45 + 50 antibody from Neomarkers.  She uses a mild CC1, the antibody
diluted 1:50 and a 32min incubation time.

Hope this helps.

What is the other D2-40 antibody?

Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of B T
Sent: Friday, 05 November 2004 8:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HMB50 and D2-40


Hi all,

Is anyone out there doing melanoma clone HMB50 on the Ventana Benchmark? If
so, what protocol are you using? I am having no success working up this
antibody and at this point i'm not sure if there might be a problem eith the
antibody itself.

Also, is anyone using D2-40 on the Benchmark......I am getting staining but
would like to punch it up a notch without having the tissue fall off the
slide.

Help please!

Barb



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


__________________________________________________________________

This electronic message and any attachments may be confidential.  If you
are not the intended recipient of this message would you please delete the
message and any attachments and advise the sender. Western Sydney
Area Health Services (WSAHS) uses virus scanning software but excludes 
any liability for viruses contained in any email or attachment.

This email may contain privileged and confidential information intended
only for the use of the addressees named above. If you are not the
intended recipient of this email, you are hereby notified that any use,
dissemination, distribution, or reproduction of this email is prohibited. If
you have received this email in error, please notify WSAHS
immediately. 

Any views expressed in this email are those of the individual sender 
except where the sender expressly and with authority states them 
to be the views of WSAHS.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet