[Histonet] embryo cryosection problem
|From:||"Anna Elisse Beaudin" |
I recently tried cryosectioning embryos taken at dpc13-17, and had a
horrible time because sections were crumbling to pieces, due to what I
believe was some really 'crusty' bone or cartiledge sticking out and
ripping the tissue as the blade passed over it (this is in the head).
I am new to working with embryos, and I am trying to figure out what I
did wrong! I fixed embryos 24-48 hours in 4% paraformaldehyde, and
then snap froze in OCT. should I have decalcified these specimens? or
cryo-protected them? I would greatly appreciate anybody's advice on
this! thanks so much in advance!
Division of Nutritional Sciences
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