[Histonet] Xylene substitute problems
From: | jo-ann-e.bader@staff.mcgill.ca |
Good morning to all histonetters,
At around May of this year I had to change from xylene to a xylene substitute
and have been hhaving problems eversince. I am processing all kinds of mouse
tissue and I find that the tissue seems to be softer, produces more wrinkles
and is generally more difficult to cut. Currently I am using 3 baths at 1 hour
each. I have been using diffrent times throughout the summer. If anyone has
any pointers I would appreciate it.
Thanks
Jo-Ann
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