I agree, but the problem with EM in this situation is 1.) polyomavirus
infection in the kidney can be extremely focal so it may be missed on EM
and 2.) clinicians may need a "same-day" result in order to adjust the
patient's immunosuppression.  How fast can you turn around your EM?

Richard


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>>  11/02/04 12:55PM >>>







Debra-
BK, Polyoma virus, can be demonstrated beautifully with Electron
Microscopy.
That is, if you have access to an EM lab.
I am frequently processing renal core biopsies to r/o BK.
If you would like assistance-
let me know.



Date: Mon, 1 Nov 2004 16:16:43 -0500
From: Browning Deb 
Subject: [Histonet] polyoma virus, SV40
To: "'histonet@lists.utsouthwestern.edu'"
             
Message-ID:

<3AADFB88753AD31189C100902786B91C0E27851F@hch_nt_exchange.hhsc.ca>
Content-Type: text/plain;            charset="iso-8859-1"

Is anyone out there using an antibody against polyoma virus, SV40; BK;
or
JC, against human tissue for demonstrating kidney rejection?  If so,
could
you share the details, thanks.

Debra Browning, ART
Technical Specialist, Immunohistochemistry
Anatomic Pathology
Hamilton Health Sciences
phone: (905) 527-4322  ext 46131
e-mail:  browning@hhs





Theresa R Dobersztyn HT ASCP
Electron Microscopy Laboratory
Department of Pathology

Akron Children's Hospital
1 Perkins Square
Akron, Ohio 44308

330-543-8279


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