[Histonet] Re: GMA sectioning
Hi Ben,
We have quite regularly cut GMA at 10, 20, 30 and 40 microns. We use a
cotton bud to apply a little water to the face of the block, just gently
rubbing it in. Also place some water on the glass knife as the section
will come off easier. Then take a section and push it to the bottom of the
water bowl/dish so it will flatten out. Then slide the sections onto the
slide. Heres the tricky part.... We put a piece of filter paper on the
bench then the slide on top, then another piece of wet filter paper on top
of the slide (and sections) and use a roller (like a paint roller)
applying a little pressure while rolling around 20-30 times over the slide
and finally place on a hotplate (not flat) that is around 60 degrees
C. Haven't had a problem. :)
Hope that makes sense.
Caroline
Caroline Stott
Histology Service Unit
University of Otago
PO Box 913
Dunedin, New Zealand
Ph (03) 479 7152
Fax (03) 479 7136
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