[Histonet] RE: p21 mAb
For p21 WAF/CIP1 we used DakoCyto antibody M7202, clone SX118. After HIER (Tris-EDTA pH9.0, 15 min 100C) the antibody stained very well at 1:25 (60 min, RT) using an EnVision+ detection with DAB+ as chromogen. As "counterstain" we performed double staining with CD31 and alpha-actin to get an idea which nuclei actually stained. Results were great when using sequential double staining: p21 - EnV+ - DAB+ - CD31 or SMA - PowerVision-GAM/AP - Liquid Permanent Red (not too dark!!) - haematoxilin (weak!!).
Hope this helps.
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center
Amsterdam - The Netherlands
----- Original Message -----
>From "Richard Cartun"
Date Mon, 01 Nov 2004 10:23:41 -0500
Subject [Histonet] p21 mAb
Is anyone doing IHC for p21 (clone EA10) on paraffin sections? If so,
where do you get it? Thank you!
Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
80 Seymour Street
Hartford, CT 06102
(860) 545-0174 Fax
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