[Histonet] Modified Gomori's Trichrome
I have been having some trouble getting the mitochondria to stain well with
this stain. It stains only slightly when made up fresh. I have tried new
reagents, low ph, No luck.
Method I use: Mayer's Hematoxylin 5 min. wash
Gomori's trichrome 10 min. wash
0.2% acetic acid 10 sec.
Gomori's: 0.6 g chromotrope 2R
0.3 g fast green
0.6 g phosphotungstic acid
1.0 ml glacial acetic acid
100 ml H2O
I just did the exact same method but used Celestine blue 2R for 5 min.
(found an old method with this step in it) before the Mayer's Hematoxylin &
the mitochondria stained up beautifully - bright red!!
Can anyone explain why this would happen or have any trick to make this
stain work without the celestine blue step.
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