Re: [Histonet] eosinophils
Dear Garry and All,
We have used the method of Dominici (1902) as quoted in Peter Gray's
Encyclopaedia to demonstrate eosinophils.
It uses an aqueous solution containing 0.5% Eosin Y and 0.5% Orange G
for 7 minutes, rinse in water
then counterstain with 0.5% aqueous Toluidine Blue O for
The other simple way to make eosinophils stand out is to do a H+E stain
but overstain with Eosin and then differentiate the eosin until other
structures, such as muscle or collagen, are very pale pink and the the
eosinophils will be prominent. The Eosin solution we use contains Eosin Y
and Erythrosin B.
Eosinophils are very satisfying to stain, particularly horse
At 08:36 AM 11/26/03 +0000, Garry Ashton wrote:
Ph 07 4781 4468
Could anybody please give me some advice on the
staining of eosinophils, either by conventional stains or
Am I right in thinking the carbol chromotrope
technique is one such method.
Many thanks in advance.
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