Re: [Histonet] eosinophils
Dear Garry and All,
We have used the method of Dominici (1902) as quoted in Peter Gray's
Encyclopaedia to demonstrate eosinophils.
It uses an aqueous solution containing 0.5% Eosin Y and 0.5% Orange G
for 7 minutes, rinse in water
then counterstain with 0.5% aqueous Toluidine Blue O for
20seconds.
The other simple way to make eosinophils stand out is to do a H+E stain
but overstain with Eosin and then differentiate the eosin until other
structures, such as muscle or collagen, are very pale pink and the the
eosinophils will be prominent. The Eosin solution we use contains Eosin Y
and Erythrosin B.
Eosinophils are very satisfying to stain, particularly horse
eosinophils.
Regards,
Laurie.
At 08:36 AM 11/26/03 +0000, Garry Ashton wrote:
Dear
all,
Could anybody please give me some advice on the
staining of eosinophils, either by conventional stains or
immuno.
Am I right in thinking the carbol chromotrope
technique is one such method.
Many thanks in advance.
Garry Ashton
PICR
UK
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Mr.Laurie
Reilly
Ph 07 4781 4468
Physiology &
Pharmacology
Fax 07 4779 1526
Aust.Inst.of Tropical Vet.& Animal Sc.
James Cook University
Townsville Qld.
4811
laurie.reilly@jcu.edu.au
Australia.
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