Re: [Histonet] aqueous mounting medium nad refractive index

From:"Bryan Llewellyn"

By better refractive index, I presume you mean higher refractive index.  If that is correct, the aqueous medium I used when I wanted good clarity was PVP glycerol.  I have no reference, nor do I know who introduced it, but the recipe is:
Polyvinylpyrrolidone  50 grams
Distilled water 50 ml
Stir the two together, and put in a 56C incubator until it is a clear syrup.  Add 10 mL glycerol, mix very well.  Leave another hour or so for bubbles to rise, then cool to room temperature.  In a capped container it will last for years.
Almost dry sections, place the medium onto the center of the section, not the edge of the slide.  Apply coverslip.  Do not press.  Make any adjustments to the coverslip immediately as it sets in about five minutes.  Its disadvantage is that it slowly dries back over some months.
I used this medium for muscle biopsy enzymes and it worked very well.
Bryan Llewellyn
----- Original Message -----
From: David Laidley
Sent: Tuesday, December 02, 2003 9:20 AM
Subject: [Histonet] aqueous mounting medium nad refractive index

Does anyone know the refractive index of Faramount (Dako) and PVA-glycerol, both aqueous mounting mediums.
Can anyone suggest an aqueous mounting medium that has a better refractive index than the ones mentioned above (if available)
David Laidley
Memorial University of Newfoundland
Division of Basic Medical Sciences
St. John's, Newfoundland

Post your free ad now! Yahoo! Canada Personals

<< Previous Message | Next Message >>