I would suggest you try genejuice from Novagen.  I get remarkably 
high transfection efficiencies with very little toxicity and have 
been able to transfect cell lines that others in my lab say are not 
transfectable.

Sharon

>Hello All
>
>Currently I am trying to transfect umbilical cord cells with GFP 
>gene , the cells are not cultures, they are in suspension , and I do 
>not want to culture them becuase I am studying their maturity level 
>and I dont want any factors to promote differentiation .
>
>I would like to know if anyone has a protocol in that regard .
>
>Thank you
>
>Hamdi Abu-Ali, MD.
>
>
>=-=-=-=-==-==-=-
>Hamdi Abu-Ali , MD.
>University of South Florida
>Cardiology - Tampa, Florida
>
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