Re: [Histonet] Stain for H.Pylori

From:"Hernan Aldana Marcos"

MODIFICATION OF GIEMSA STAIN FOR HELICOBACTER

FIXATION: Neutral Formolin   10%

SECCIONS: 4-5 µm

 

SOLUTIONS:

ACETIC WATER

Acetic acid............... 0,01 ml

Destilated water............... 1000,00 ml

 

Working solution GIEMSA

Giemsa.............. 10,0 ml

Acetic water.............. 50,0 ml

 

PROCEDDURE

1.     Take slides to water destiled

2.     Giemsa working solution 1 h

3.     Deshidratation in 3 changes of alcohol 100

4.     xilol, syntetic mounting medium

 

RESULTADOS

H. pylorii.............. intense blue

Nuclei.............. blue

Cytoplasm.............. Rose

 

REFERENCE

Luna L.: "Wet work shop for special Alabama" Noviembre 2 de 1984
 
 
Dr. Hernán J. Aldana Marcos
Facultad de Medicina. Universidad de Morón
Machado 914. B1708JPD. Buenos Aires. Argentina
e-mail alternativo hernanjavier@yahoo.com
web: http://hjaldanamarcos.bravepages.com
http://histologia.bigthicketdirectory.net/main.html
----- Original Message -----
From: Jill Cox
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, December 03, 2003 1:58 PM
Subject: [Histonet] Stain for H.Pylori

Hello everyone,
 Has anyone done a Toluidine blue/Alcian yellow stain for H. Pylori? If so how do the Doctors like it?   Or, what other stains are you doing for that. I am trying to get away from the Genta!! Thanks in advance


Jill Cox HT (ASCP)
Histology Supervisor
Seattle Histology Lab


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