Re: [Histonet] LOST OF ANTIGENICITY
Yes antigenicity can be lost on slides. I recommend that you cut 25
slides test the first and last then keep a log . When the stainging starts
to decrease, that will tell you the life of your slide antigenicity.
Temp and climate effect slides in a lab as well as fixation and processing
of specimens. Thats one reason controls are to be obtained from with in
one's lab. If labs send out immunos,if or ish control tissue should be sent
from the lab to the reference to be completely accurate for the test to be
St. Mary Hospital
>From: email@example.com (Anne-Sophie Martinez)
>Subject: [Histonet] LOST OF ANTIGENICITY
>Date: Tue, 25 Nov 2003 17:33:50 +0000
>I do immunofluorescence with an AB against one aquaporine (AQP - water
>channel) and mdrA (multi-drug resistance protein) on some fish tissues
>fixed in paraformaldehyde or Bouin, embedded in paraffin and even sometimes
>cut (1 or 3 years ago!!).
>I can't manage to have any fluorescence even with very high concentrations
>of AB. Do you think that it can be due to the age of the tissues? Can the
>tissues loose the antigenicity when they have been embedded and/or cut for
>such a long time? Does anybody have an idea of an other possibility?
>Dr Anne-Sophie Martinez
>School of Biology
>University of St. Andrews
>Bute Medical Buildings
>Fife, KY16 9TS
>Tel + 44 (0) 1334 463546
>Fax + 44 (0) 1334 463600
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