I have used hypoxyprobe in mice GBM model, works well but you may want to
stay away from peroxidase if there's a lot of necrosis.  Might also want to
consider antibody against hif1alpha.....
Luis

-----Original Message-----
From: histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of danyl002
Sent: Tuesday, December 02, 2003 9:37 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Hipoxyprobe for retina


 Did anyone use Hipoxyprobe staining for retina or optic nerve? Is there
any other way to measure a degree of hypoxia in nerve tissue(besides actual
axon counting)?



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