RE: [Histonet] Citrate Buffer pH

From:"Patsy Ruegg"

Has anybody tried the AR reagents that deparaffinzed such as BioCare Decloaker in a steamer in place of the pressure cooker?  PC is too harsh for some of my stuff but I like using these retrieval/deparaffination reagents.



-----Original Message-----
From: []On Behalf Of Jackie.O'
Sent: Tuesday, December 02, 2003 2:50 PM
To: Pat Flannery
Subject: Re: [Histonet] Citrate Buffer pH


I've been using a product from Biocare, a Universal Decloaker (6.0 citrate buffer) , which deparaffinizes during HIER in their pressure cooker (Decloaking Chamber) - they are a very Trekkie oriented company.  It works great - saves a bunch o' steps.  You might try contacting them for the specifics.  I don't think about the nature of these things if they work - I just follow along blindly . . . . . . . . .sometimes.

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics


Pat Flannery <>
Sent by:

12/02/2003 03:00 PM

        Subject:        [Histonet] Citrate Buffer pH

I have a procedure for deparaffinizing (their word, not mine) tissue
sections in order to do immunostaining using the Vector MOM kit.  It
calls for heating the section (briefly) in a microwave in 0.1M Citrate
Buffer.  The procedure  does not specify the pH of the buffer, just to
make it by mixing 0.1M NaCitrate and 0.1M citric acid.  Does this sound
familiar to anyone out there?


-Patrick J. (Pat) Flannery
Division of Nephrology (that's kidneys to you)
Box 3014 (that's NOT "PO" just "Box")
Duke University Medical Center
Durham, NC  27710
E-mail: (preferred)
        FLANN002@MC.DUKE.EDU (also works)
Voice:   (919)660-6863
Fax:     (919)684-2929

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