AFAIK, the usual pH is 6.0

Min-han Tan

-----Original Message-----
From: Pat Flannery [mailto:pjfnefro@duke.edu] 
Sent: Tuesday, December 02, 2003 4:01 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Citrate Buffer pH


I have a procedure for deparaffinizing (their word, not mine) tissue 
sections in order to do immunostaining using the Vector MOM kit.  It 
calls for heating the section (briefly) in a microwave in 0.1M Citrate 
Buffer.  The procedure  does not specify the pH of the buffer, just to 
make it by mixing 0.1M NaCitrate and 0.1M citric acid.  Does this sound 
familiar to anyone out there?

-- 

-Patrick J. (Pat) Flannery
 Division of Nephrology (that's kidneys to you)
 Box 3014 (that's NOT "PO" just "Box")
 Duke University Medical Center
 Durham, NC  27710
 E-mail: pjfnefro@duke.edu (preferred)
         FLANN002@MC.DUKE.EDU (also works)
Voice:   (919)660-6863
Fax:     (919)684-2929




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