Robbert Rottier,
Yes, I did. It is hard to judge your how highly
expressed mRNA, which Maybe I also  only can detect.  
It is reasonable something not high enough to be
detected.  For example, our experiement is to
electroperate some gene expression vector to one side
of some embryo brain (this is chick), after some
while, we detect the change of some other gene
expression.  mightbe the control side no detectable
sign and the experiement side has. and there is timing
 gene expression.  Your question beyond my ability. 
If you can overcome this problem, please kindly let me
know.
Zhimin Lao

--- "R. rottier"  wrote:
> Dear Zhimin Lao,
> 
> 
> I read your comments zbout in situs on slides in the
> histonet list. Have
> you tried to do is situs with DIG probes on paraffin
> embedded material?
> Our experience is that only highly expressed mRNAs
> can be detected.
> 
> What is your experience??
> 
> Sincerely,
> 
> Robbert Rottier



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