[Histonet] Re:Old sections, and BM ISH/IHC


Excuse me, my first note and the system will not let me open the mail to use to reply, anyway

We found that the heat mediated epitope retrieval largely obviated problems with sections that had been hanging around for months, being reviewed etc, or archival blocks fron ten or more years ago

Bone marrow,  well a couple of thoughts and questions.  Fixing smears for IHC has been 30 mins in Neutral Buffered Formalin, could look for the cytology paper that said as much.   So we used the same for any Bone marrows we get, once in a blue moon.  ISH, no experience, but..   I am looking for thoughts on BM Trephine biopsies. When we do ISH then the results appear much weaker than the tonsil or other tissue that runs alongside.  That is whether we are enzyme or heat mediated pretreatments.   Any ideas?    Trephines are EDTA decalcified.  IHC is no problem

David Edmondson
Christie Hospital
Manchester  UK

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