[Histonet] RE: transfecting GFP into suspension cells
You asked about getting a GFP construct into cells growing in suspension -
2 possibilities:
If you have access to the proper "toy", you can electroporate. But you'll
need an electroporator and the cuvettes....and working out the conditions
for a new cell line can be a royal pain.
Second option - use one of the transfection reagents available on the
market. FuGene and Lipofectamine are the "old standards"; check with
Clontech, Roche, Gibco....
Good luck!
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Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward@unm.edu
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