[Histonet] Nuclear staining artefact
It never fails, just when I start to think that I
have everything worked out in Immunohistochemistry I find something new to
prove that I still have a lot to learn. I have begun to see some strange nuclear
staining in bone marrow biopsies. There isn't a general increase in background
only the specific nuclear staining in the negative control. I have seen
this before, but usually only the rare cell and only on the periphery of
the tissue. Recently, however, the tissues have shown a large number
of positive staining nuclei throughout the tissue. This artefact doesn't
show up in the slides stained with a primary antibody only the negative control.
I have never seen this in any tissue other than bone marrow biopsies. We
fix our bone marrows in B5 and decalcify with RDO. Has anyone else seen this?
Does anyone have a theory? Thanks. Tom
Lions Gate Hospital
North Vancouver, BC
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