[Histonet] GFP mounting media

From:"Rebecca D. Riba"


I have been reading the list for recommendations on mounting media used
for tissues expressing GFP.  I have seen arguments on PBS versus antifade
medias (vectashield, prolong, homemade, etc.).  In addition, discussion of
the use of fresh frozen, OCT frozen, and paraffin. Does anyone have any
additional recommendations on these protocols for visualizing egfp or
dsred expressing tissues. In addition, has anybody used vectashields
hard-set mounting media?

Thank you,
Rebecca Riba
Weill Medical College of Cornell

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