[Histonet] Double immunostaining
Thanks all for the
very considered answers to my last question.
1. I would
like to enquire - I am planning on using rhodamine and FITC to double stain my
tissue slides with anti-p21 and anti-p27. Unfortunately, both are nuclear=20
proteins, and I have heard horror stories from a couple of colleagues on the
difficulties of staining the same structure with different dyes. According to
them, usually only one dye is predominant. Does anyone have any
experiences attempting to stain the nucleus with two different dyes, or would
the recommended approach be to stain two adjacent sections?
2. I have noted an
apparent artefact during my ABC-DAB immunohistochemical staining of tissue. The
tissue border stains heavily (an obvious artefact), but just below the border,
there is a rim which completely doesn't stain at all, in stark contrast to the
centre of the section. Has anyone else experienced this?
This email message, including any attachments, is for
the sole use of the intended recipient(s) and may contain confidential
information. Any unauthorized review, use, disclosure or distribution is
prohibited. If you are not the intended recipient(s) please contact=20the
sender by reply email and destroy all copies of the original message.
<< Previous Message | Next Message >>