[Histonet] Caspase-3, antigen retrieval
I am having trouble keeping human tissue on slides undergoing antigen
retrieval during Caspase-3 immunohistochemistry.
The antigen retrieval involves boiling the slides in a 0.1M Tris-HCl
buffer for 20mins in the microwave, which is causing the tissue to
fall off the slides! I have tried wrapping slides with gauze, and
using different coated slides, but so far no luck.
I am looking for a different method of antigen retrieval other than
the above, or treating the tissue with trypsin which is too harsh.
Any suggestions would be most welcome!!
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