Re: Re: [Histonet] Trichromes

From:"Hernan Aldana Marcos"

Two papers are wonderful in relation to the biological staining. The fisrt "A contribution to the theory of biological staining based on the principles of structural organization of biological macromolecules " Biotechnic and Histochemistry, 2001, 76(3): 137-161. This explain the posible form of binding to collagen in trichormes and other staining formulas. Is a complex paper but is superb.
The second "Van Gieson´s picrofuchsin. The staining mechanisms for collaegen and cytoplasm, and examination of the dye diffusion rate model of differential staining". Histochemistry (1993). 99: 163-174 is related of the staining mechansims of van Gieson´s stain technique.
Both papers were made by Poul Prento, I contact him several times for some dudes related with staining methos and he always answer me in a perfect form. Do it, write to him
the mail is  
 Sorry for my bad English
Dr. Hernán J. Aldana Marcos
Facultad de Medicina. Universidad de Morón
Machado 914. B1708JPD. Buenos Aires. Argentina
e-mail alternativo
----- Original Message -----
From: Ian Montgomery
Sent: Monday, November 17, 2003 9:47 AM
Subject: Fwd: Re: [Histonet] Trichromes (was Re:What is Histology?)

        Can I recommend you obtain a copy of Histological & Histochemical Methods (Theory and Practice) by J.A. Kiernan. It's full of the referenced information you require.

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Subject: Re: [Histonet] Trichromes (was Re:What is Histology?)
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Ok guys i am sorry i was not clear initially on my request.Here is what I need,I am looking for a research paper type in which compares the different types of staining procedures and there uses.The two stains of interests are the Trichrome and the Alcian Blue.The paper must include the history of how the stain was introduced and the mechanism by which it works and lastly, for what purposes is the stain used.Idont know how anyone can write a 10 page paper on such,but that is the assignment I have and do not know how to proceed with this.Thanks


Dr. Ian Montgomery,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883

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