A vibrating microtome may be superior for this - or you have to warm up the
cryostat chamber in order to cut at warmer temperatures. 

At 02:29 PM 11/13/2003 -0600, you wrote:
>I would appreciate any suggestions on cutting 100 micron frozen sections of 
>mouse brain for immunohistochemistry.  our sections either crack or show a 
>lot of chatter.
>
>
>Thanks,
>
>Cora Bucana
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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