Re: [Histonet] separated cells

From:Gayle Callis

>Do you mean by a perfect sheet of cells, a perfectly flat paraffin
section?  When you speak of sheet of cells, I envision cell cultures with a
sheet of cells attached to bottom of a culture plate. 
>At 12:11 PM 11/20/2003 -0500, you wrote:
>>Dear Histonet,
>>My Pathologist would like to see a perfect sheet of cells. He wants the 
>>vacuoles and smashed sells eliminated.  We have tried many different 
>>processing programs and have changed the temperature of the water on the 
>>stainer.  I have sent pictures of the problems with formalin fixed skin
>>at 4 microns) and a bone marrow cut at 3 microns)  If anyone has any 
>>suggestions I would be very grateful.  The pictures are listed as separated 
>>cells 1 & 2 @ histonet pictures.
>>Bernadette Weston, HT
>>Histology Supervisor
>>Barberton Citizens Hospital
>>barberton OH
>>Histonet mailing list
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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