You are right that might be a problem, but with tissuearrays you cannot re-embed the specimens. All the cores would float around and make a big mess. Specimens would be mixed causing the entire array to be unusable. If the cores are loose from not setting the punches properly in the first place, the only thing Jason could do is again place the array block on a glass slide and re-warm the block for another 15 to 20 minutes at 37-40 degrees Celsius. Then press the array block to a glass slide again to reset the punches. Then cool them on an ice tray to remove the glass slide.
Also warming the array block would also help to set any paraffin that has separated from a shell like problem. If that were his problem, since he cannot re-embed an array.
Thom Jensen
For more information on Tissue Microarray instruction visit: www.arrayworkshop.com
>From: "louise renton"
>To: kosmicdog@hotmail.com, histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] sectioning tissue mircroarrays
>Date: Mon, 10 Nov 2003 09:02:26 +0000
>
>Dear Jason,
>
>Could it be that when the cores were insetrd into the final block
>they were not warmed sufficiently? I have seen this "shelling" out
>of normal tissue bocks when there has been a temperature mismatch,
>ie when the block of tissue was cold and a hardened layer of wax had
>formed, and then embedded in normal hot wax. The only remedy under
>these circumstances was to melt the whole thing down and start
>again.
>
>best regards
>
>
>
>Louise Renton
>Bone Research Unit
>MRC
>Johannesburg
>South Africa
>Tel & fax +27 11 717 2298
>"Time flies like an arrow, fruit flies like a banana"
>
>
>
>
>
>----Original Message Follows----
>From: "jason madore"
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] sectioning tissue mircroarrays
>Date: Fri, 07 Nov 2003 06:54:18 -0800
>
>Salut everyone,
>
>I am hoping that someone can give me pointers for sectioning of
>tissue microarrays. I am using leica RM2125 and the first array i
>need to section is a 0.06mm 400core ovarian serous tumour array. I
>have a problems with the cores rolling up as the section is cut.
>About half of the roled cores flatten out in the water bath but
>still losing half the array in this way is not good. Is the knife or
>some step prior to sectioning. The block was incubated at 37ds for
>half an hour. Any pointers on dealing with these problems or any
>other problems I might encounter would be greatly appreciated.
>Thanks in advance. I am of course checking the histonet archives as
>well.
>
>Ciao, J.
>
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