Re: [Histonet] sectioning tissue mircroarrays

Jason,

I found that dulling the blade with a Kimwipe knocks the edge off the blade down enough to get ribbons right away. Try not to soak the array block in water. If so, only for a few minutes - use straight ice. Sometimes the block will cut better once it gets almost to room temp. Also, if you are noticing any lines in the paraffin move to a new part of the blade or change to a new blade.

Thom

For more information on Tissue Microarray instruction visit: www.arrayworkshop.com

>From: "jason madore"

>To: histonet@lists.utsouthwestern.edu

>Subject: [Histonet] sectioning tissue mircroarrays

>Date: Fri, 07 Nov 2003 06:54:18 -0800

>Salut everyone,

>I am hoping that someone can give me pointers for sectioning of

>tissue microarrays. I am using leica RM2125 and the first array i

>need to section is a 0.06mm 400core ovarian serous tumour array. I

>have a problems with the cores rolling up as the section is cut.

>About half of the roled cores flatten out in the water bath but

>still losing half the array in this way is not good. Is the knife or

>some step prior to sectioning. The block was incubated at 37ds for

>half an hour. Any pointers on dealing with these problems or any

>other problems I might encounter would be greatly appreciated.

>Thanks in advance. I am of course checking the histonet archives as

>well.

>Ciao, J.

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