Re: [Histonet] heart IHC work

From:Gayle Callis

Cut frozen sections, then fix for IHC.  Why compromise the tissue twice,
frozen then thaw, fix and process?  That is how I interpret this message,

At 02:13 PM 11/11/2003 -0700, you wrote:
>I am involved in a project that requires doing IHC for Lamin A/C on heart
>tissue.  If anyone has experience with this please tell me all you know.
>Also, since most of this is DNA work they have pieces of fresh heart frozen
>at -70 dC.  What would be the best way to fix and process the frozen heart
>so that it would be well preserved for IHC.
>Thank you for advise in this matter
>Histonet mailing list
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

Histonet mailing list

<< Previous Message | Next Message >>