Bless you!  Thank goodness, and thank YOU (& Histonet!) for not having to
reinvent the wheel!  I'm very grateful to be able to benefit from your
experience!  with my sincere appreciation,    Susan
----- Original Message ----- 
From: "Houston, Ronnie" 
To: "'sebres'" 
Sent: Thursday, November 06, 2003 8:12 AM
Subject: RE: [Histonet] ethopropazine/acetylcholinesterase histochemistry


>
> Susan,
>
> We used to use iso-OMPA (can't remember it's full name now) to inhibit
> non-specific esterases when I worked in neuropathology many moons ago.
> However, by its very nature (an extremely potent cholinesterase inhibitor)
> we decided to try it without the inhibitor present. Almost impossible to
> notice the difference!!!  Personally, with the inherent risks involved, I
> would do without.
>
> Ronnie
>
> Ronnie Houston
> Regional Histology Operations Manager
> Bon Secours HealthPartners Laboratories
> 5801 Bremo Road
> Richmond, VA 23226
> (804) 287 7972
> ronnie_houston@bshsi.com
>
>
> -----Original Message-----
> From: sebres [mailto:sebres@comcast.net]
> Sent: Wednesday, November 05, 2003 6:36 PM
> To: Histonet (E-mail)
> Subject: Re: [Histonet] ethopropazine/acetylcholinesterase
> histochemistry
>
>
> Well, I've already dug a little deeper & come up with part of the answer,
> but am still in need of advice:  I understand better now that it is
> specifically butyrylcholinesterase that ethopropazine is supposed to
> inhibit.  I've found some evidence that ethephon is considered a specific
> butyrylcholinesterase inhibitor, & this is actually available for purchase
> from agricultural supply companies.  Any thoughts out there on whether
this
> sounds like a viable solution?   Many thanks,  Susan
> ----- Original Message ----- 
> From: "sebres" 
> To: "Histonet (E-mail)" 
> Sent: Wednesday, November 05, 2003 6:03 PM
> Subject: [Histonet] ethopropazine/acetylcholinesterase histochemistry
>
>
> > I'm teaching a neurohistology class in a research university, mainly
Nissl
> > staining, immunohistochemisty & in situ hybridization histochemistry on
> rat
> > brain sections.  I thought I'd add to the mix a good old fashioned
enzyme
> > histochemistry assay, such as the elegant acetylcholinesterase method
> > described in Paxinos & Watson's Rat Brain atlas, which sounds
refreshingly
> > simple.  But, to my shock, ethopropazine, since it is now used
medicinally
> > (Parsitan), seems to no longer be available except by prescription!  If
I
> > understand this correctly, the main purpose of this reagent in this
assay
> is
> > as a cholinesterase inhibitor, in which case I'm wondering whether it
> might
> > work to substitute either physostigmine, or possibly haloperidol, both
of
> > which I already have in hand?  My students and I would all be extremely
> > grateful for any advice about this!     Susan Bachus, George Mason
> > University
> >
> >
> > _______________________________________________
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> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
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