Re: [Histonet] ethopropazine/acetylcholinesterase histochemistry


Well, I've already dug a little deeper & come up with part of the answer,
but am still in need of advice:  I understand better now that it is
specifically butyrylcholinesterase that ethopropazine is supposed to
inhibit.  I've found some evidence that ethephon is considered a specific
butyrylcholinesterase inhibitor, & this is actually available for purchase
from agricultural supply companies.  Any thoughts out there on whether this
sounds like a viable solution?   Many thanks,  Susan
----- Original Message ----- 
From: "sebres" 
To: "Histonet (E-mail)" 
Sent: Wednesday, November 05, 2003 6:03 PM
Subject: [Histonet] ethopropazine/acetylcholinesterase histochemistry

> I'm teaching a neurohistology class in a research university, mainly Nissl
> staining, immunohistochemisty & in situ hybridization histochemistry on
> brain sections.  I thought I'd add to the mix a good old fashioned enzyme
> histochemistry assay, such as the elegant acetylcholinesterase method
> described in Paxinos & Watson's Rat Brain atlas, which sounds refreshingly
> simple.  But, to my shock, ethopropazine, since it is now used medicinally
> (Parsitan), seems to no longer be available except by prescription!  If I
> understand this correctly, the main purpose of this reagent in this assay
> as a cholinesterase inhibitor, in which case I'm wondering whether it
> work to substitute either physostigmine, or possibly haloperidol, both of
> which I already have in hand?  My students and I would all be extremely
> grateful for any advice about this!     Susan Bachus, George Mason
> University
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