Re: [Histonet] Trichromes (was Re:What is Histology?)
Your difficulty is going to be keeping the paper to
only 10 pages on each of the stains!
The textile dye, Alcian blue 8Gx was formulated by
Haddoch and Wood in 1944 and introduced as a histological stain by Steedman in
Alcian blue can be used, either alone or in
polychromatic mixtures, for a variety of staining methods. These range from
the demonstration of amyloid to assorted glycosaminoglycans.
As pointed out by John Kiernan, the trichrome
methods are very numerous and varied in their application.
You probably are most interested in those methods
used for the demonstration of supporting(connective) tissue
Most likely the first of these was the single
solution containing two anionic dyes introduced by Van Gieson in
Today many people use the Masson (1929) technique
in which two anionic dyes are applied sequentially, but separated by a
heteropolyacid such as PTA.
The first of many triacid stains was probably that
of Mallory in 1900.
I would recommend that you obtain the following
texts from the library;
The History of Staining, 3rd ed.,Clark and
Kasten,Willams and Wilkins. ISBN 0-683-01705-5,
Histopathological Technique and Practical
Histochemistry, 3rd ed., R D Lillie, McGraw-Hill. Library of
Congress Catalog Card Number: 64-7866
Histological and Histochemical Methods, 3rd ed.,
J.A.Kiernan, Butterworth Heinemann. ISBN 0 7506 4936 4
----- Original Message -----
Sent: Monday, November 17, 2003 6:57
Subject: Re: [Histonet] Trichromes (was
Re:What is Histology?)
Ok guys i am sorry i was not clear initially on my
request.Here is what I need,I am looking for a research paper type in which
compares the different types of staining procedures and there uses.The two
stains of interests are the Trichrome and the Alcian Blue.The paper must
include the history of how the stain was introduced and the mechanism by which
it works and lastly, for what purposes is the stain used.Idont know how anyone
can write a 10 page paper on such,but that is the assignment I have and do not
know how to proceed with this.Thanks
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