Re: [Histonet] Help on Teared tissues

From:Deanne Catmull

Hi Julien,

According to my troubleshooting protocol it says that either poor
processing of tissue or the wax being too hot during infiltratiuon or
embedding is probably your problem.

Deanne at La Trobe Uni.
At 08:28 PM 11/7/03 +0000, Julien Lambrey de Souza wrote:
>Hello all,
>Could someone give me the various reasons why tissues are torn on slides. In 
>my case I doubt that this is due to the microtome. Is processing in parafin 
>a possibility? Maybe longer times in 100% alchool, Xylen and then parafin?
>Julien De Souza.
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Deanne Catmull	
Neuroimmunology Laboratory
Dept of Biochemistry
La Trobe University
Bundoora Vic 3086

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