Re: [Histonet] Distinguishing monocytes from granulocytes

From:Mark Frei


Have you thought of cytochemistry?
Chloroacetate esterase and naphthyl acetate esterase are two enzymatic assays that will easily and cheaply differentiate the mentioned cell types.


Ramesh Subrahmanyam <ramesh@brandeis.edu>
Sent by: histonet-admin@lists.utsouthwestern.edu

11/05/2003 11:46 AM

       
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        Subject:        [Histonet] Distinguishing monocytes from granulocytes



Hello Histonetters,
I am trying to distinguish immature monocytes from immature granulocytes
in Balb/c mice. Can anyone suggest antibodies for FACS or
immunohistochemistry or RT-PCR markers that would help in this?
Suggestions on other methods would be welcome too.
Thanks in advance,

Ramesh
Brandeis University
Massachusetts


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