Re: [Histonet] Decreased antigenicity with alcohol-formalin fixation
diana, I use penfix on my processor and we have good luck with our ers and
prs, we also use it sometime to fix breast tissue before going on the
processor. haven't had any problems with it. hope this helps.
>From: "Goodwin, Diana"
>To: "Histonet (E-mail)"
>Subject: [Histonet] Decreased antigenicity with alcohol-formalin fixation
>Date: Fri, 14 Nov 2003 11:39:21 -0500
> > Greetings, Histonetters.
> > One of my pathologists has expressed concern that antigenicity for
> > antibodies, specifically ER and PR, in breast tissue that is processed
> > using alcohol-formalin will be decreased. Can anyone cite a reference
> > the literature that confirms or addresses this concern? Has anyone had
> > this experience, or does anyone know of any reason that using
> > alcohol-formalin (specifically Pen-Fix) would adversely affect IHC
> > staining?
> > Thanks to all of you,
> > Diana Goodwin
> > Supervisor, Anatomic Pathology
> > Preston 6
> > 215-829-6532
> > e-mail: firstname.lastname@example.org
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