Re: [Histonet] DBA staining for parietal cells
There is a fabulous book on Lectin staining available through Springer
Verlag, or Bios Scientific publisher.
This book is a tell all on lectins plus has wonderful immunostaining
protocols in general.
Also go to Vectorlabs website and look at their lectin information. They
tell you how to do a negative control, plus give the sugar that would be
used for that control with each lectin.
One has to be a bit careful when working with lectins as some will bind to
other components, so high concentration of Lectin or long incubation
periods should be avoided.
There is a lectin buffer that some lectins require. This book has the
buffer recipe. It is a good investment, if anything, just for the
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
o thank you all for helping me
> with my T and B cell staining problem. I got many replies and am working
>out some new protocols now. Thanks! has someone already worked with the
>lectin DBA (horse gram) to color parietal cells? Could you give me some
>more information about that (what are the bindingplaces of this lectin on
>the parietal cell?) I have not worked with lectins yet. Thanks!
>Manuelle Manuelle De Bock, dierenarts (DVM)
>Laboratory of Veterinary Pathology
>Department of Pathology, Bacteriology and Avian Diseases
>Faculty of Veterinary Medicine
>B9820 Merelbeke - Belgium
>Tel 0032(0)9 264 7745
>Fax 0032(0)9 264 7789
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