Re: [Histonet] CD 4/CD 8 staining of frozen stomach sections of mice

Hello Manuelle

may I ask you to check your secondary antibody? How does the control look like without applying the primary ?
We use both for CD4 and for CD8a on mouse frozens antibodies( host rat)  from Pharmigen.  As secondary I use a rabbit anti-rat biotinylated one, mouse adsorbed from Vector.
For the blocking of endogenous peroxidase don't use methanol (!) , it weakens the signal in most cases. For the unspecific binding block we use 10% normal rabbit serum (-> secondary host species).
I hope this helps.
Good luck,

Antje Marcantonio
Novartis Pharma AG
BU Transplantation Research
Basel, Switzerland
Tel. +41 61 324 6730

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