Re: [Histonet] Anti-beta-galactosidase antibodies

From:Sharon Cooperman

No, sorry.  Just out of curiousity, why do you want to do both the 
enzymatic and IHC?  Also, I think that if the enzyme isn't  destroyed 
by the reaction it might work - eg., I wouldn't expect it to work on 
peroxidase, but I don't know if beta gal activity persists after the 


>Have you used this antibody on previously X-gal reacted tissue?  We 
>are using 40-1a.  Thanks, Maureen Loomer, MS candidate at ECU
>-----Original Message-----
>From: Sharon Cooperman <>
>Sent: Tue, 18 Nov 2003 18:34:04 -0500
>Subject: Re: [Histonet] Anti-beta-galactosidase antibodies
>Hi.  Chemicon AB1211, ABC kit, HRP with DAB on formalin fixed
>paraffin embedded tissue.  We used it on a transgenic mouse
>expressing Beta gal - pretty weak but detectable with ABC kit and
>HRP.  I think most of the transgenics express weakly and I would
>consider using tyramide (TSA) if you need more signal because
>background is very low.  I also think some of the Abcam anti-beta gal
>abs are pretty good (I don't know which ones, I borrowed them).
>>Has anyone had any experience with polyclonal antibody against
>>b-galactosidase? What reporter system have you used? Thank you.
>>Tom Kuzniar
>>Histonet mailing list
>Sharon Cooperman      	     <>
>NIH, NICHD, CBMB                     301.435-7735
>Building 18T, room 101               301.402-0078 fax
>Bethesda, MD 20892
>Histonet mailing list

Sharon Cooperman       	     
NIH, NICHD, CBMB                     301.435-7735
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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