Re: [Histonet] Anti-beta-galactosidase antibodies

Have you used this antibody on previously X-gal reacted tissue?  We are using 40-1a.  Thanks, Maureen Loomer, MS candidate at ECU

-----Original Message-----
From: Sharon Cooperman <>
Sent: Tue, 18 Nov 2003 18:34:04 -0500
Subject: Re: [Histonet] Anti-beta-galactosidase antibodies

Hi.  Chemicon AB1211, ABC kit, HRP with DAB on formalin fixed 
paraffin embedded tissue.  We used it on a transgenic mouse 
expressing Beta gal - pretty weak but detectable with ABC kit and 
HRP.  I think most of the transgenics express weakly and I would 
consider using tyramide (TSA) if you need more signal because 
background is very low.  I also think some of the Abcam anti-beta gal 
abs are pretty good (I don't know which ones, I borrowed them).


>Has anyone had any experience with polyclonal antibody against
>b-galactosidase? What reporter system have you used? Thank you.
>Tom Kuzniar
>Histonet mailing list

Sharon Cooperman       	     <>
NIH, NICHD, CBMB                     301.435-7735
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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