Re: [Histonet] Anti-beta-galactosidase antibodies
Hi. Chemicon AB1211, ABC kit, HRP with DAB on formalin fixed
paraffin embedded tissue. We used it on a transgenic mouse
expressing Beta gal - pretty weak but detectable with ABC kit and
HRP. I think most of the transgenics express weakly and I would
consider using tyramide (TSA) if you need more signal because
background is very low. I also think some of the Abcam anti-beta gal
abs are pretty good (I don't know which ones, I borrowed them).
>Has anyone had any experience with polyclonal antibody against
>b-galactosidase? What reporter system have you used? Thank you.
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