RE: [Histonet] thick frozen sections

From:"Alan Bright"

-Dear Cora,

Your results indicate that the mouse brain is too cold, to rectify
section @ -8 to -12C and cool the knife at least 10 colder, if a
specimen temperature control is not fitted achieve this with solid CO2
in contact with the knife. We achieve sections up to 600 microns using
this method with ease.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright@brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
From: Corazon D. Bucana [mailto:bucana@audumla.mdacc.tmc.edu] 
Sent: 13 November 2003 20:29
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] thick frozen sections


I would appreciate any suggestions on cutting 100 micron frozen sections
of 
mouse brain for immunohistochemistry.  our sections either crack or show
a 
lot of chatter.


Thanks,

Cora Bucana


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