RE: [Histonet] thick frozen sections

From:"Charles W. Scouten, Ph.D."

I agree, on both statements.  Chatter or cracking is usually too cold cutting temperature.  But freezing breaks cell membranes and leaks contents regardless.  If your target protein is in the cytosol, you will much better results without the freezing, sectioning at 100 microns is easy with a vibrating microtome.  See the link below

Charles W.  Scouten, Ph.D. 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 

-----Original Message-----
From: Gayle Callis [] 
Sent: Thursday, November 13, 2003 5:42 PM
To: Corazon D. Bucana;
Subject: Re: [Histonet] thick frozen sections

A vibrating microtome may be superior for this - or you have to warm up the
cryostat chamber in order to cut at warmer temperatures. 

At 02:29 PM 11/13/2003 -0600, you wrote:
>I would appreciate any suggestions on cutting 100 micron frozen sections of 
>mouse brain for immunohistochemistry.  our sections either crack or show a 
>lot of chatter.
>Cora Bucana
>Histonet mailing list
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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