RE: [Histonet] separated cells

From:"Marshall Terry Dr, Consultant Histopathologist"

I  can't understand this post. Is it just me?

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire

Dear Histonet,

My Pathologist would like to see a perfect sheet of cells. He wants the 
vacuoles and smashed sells eliminated.  We have tried many different 
processing programs and have changed the temperature of the water on the 
stainer.  I have sent pictures of the problems with formalin fixed skin (cut 
at 4 microns) and a bone marrow cut at 3 microns)  If anyone has any 
suggestions I would be very grateful.  The pictures are listed as separated 
cells 1 & 2 @ histonet pictures.

Bernadette Weston, HT
Histology Supervisor
Barberton Citizens Hospital
barberton OH

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